Product Info Summary
SKU: | A02409-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-RAB7A Antibody Picoband™
SKU/Catalog Number
A02409-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RAB7A Antibody Picoband™ catalog # A02409-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RAB7A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02409-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RAB7A recombinant protein (Position: A156-R197).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02409-2 is reactive to RAB7A in Human, Mouse, Rat
Applications
A02409-2 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
23 kDa
Calculated molecular weight
23.49kDa
Background of RAB7A
Ras-related protein Rab-7a is a protein that in humans is encoded by the RAB7A gene. RAB7A functions as a key regulator in endo-lysosomal trafficking, governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positions, and endosome-lysosome transport through different protein-protein interaction cascades. Furthermore, RAB7A is involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes through modulation of SOX10 and the oncogene MYC. Mutations in the lysosomal pathway result in tumor progression in melanoma cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RAB7A using anti-RAB7A antibody (A02409-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: rat L6 whole cell lysates,
Lane 4: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB7A antigen affinity purified polyclonal antibody (Catalog # A02409-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB7A at approximately 23 kDa. The expected band size for RAB7A is at 23 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RAB7A (Source: Uniprot.org, NCBI)
Gene Name
RAB7A
Full Name
Ras-related protein Rab-7a
Weight
23.49kDa
Superfamily
small GTPase superfamily
Alternative Names
CMT2B; FLJ20819; member RAS oncogene family; PRO2706; PSN; RAB7; Rab7a; RAB7A, member RAS oncogene family; Ras-associated protein RAB7; ras-related protein Rab-7a RAB7A CMT2B, PRO2706, RAB7 RAB7A, member RAS oncogene family ras-related protein Rab-7a|RAB7, member RAS oncogene family|Ras-associated protein RAB7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB7A, check out the RAB7A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB7A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAB7A Antibody Picoband™ (A02409-2)
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No publications found for A02409-2
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Customer Reviews
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